ｃｈｅｎ先生，お疲れ様でした。

本当に，しっかりと仕事をしてくれました。

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中国医科大学には，大槻から，以下を報告しておきます！！

Dr, Ying Chen have investigated the alteration of function of the CD4+25+FoxP3+ regulatory T cell caused by exposure to asbestos supervised by Prof. Takemi Otsuki, Department of Hygiene, Kawasaki Medical School, Kurashiki, Japan. The laboratory researches the immunological effects of asbestos and silica on the human immunocompetent cells. Dr. Chen is involved in the part of analyses for the effects of chrysotile on T cells. She have worked and learned human cell culture, gene expression using real-time RT-PCR method, protein expression using immunoblotting and flowcytometry, as well as immunological assays using flowcytometry. More precisely, she learned staining human lymphocytes by immunofluorescent labeled antibodies, handling flowcytometry, sorting specific cells using flowcytometry and also bead-based method, analyzing cell proliferation using growth curves, DNA incorporation using radioactive 3H-Thymidine, chromogenic methods using anti-BrdU antibody, and CFSE labeled flowcytomrtrical procedure. She also studied the functional assay of activation of effecter T cells and regulatory T cells using mixed lymphocyte reaction, and maturing the dendritic cells and dendritic cell-based T cell stimulation assay, and stimulation using anti-CD3 and CD28 antibodies. She worked on modified assays of above-mentioned functional studies using transwell method, too. In addition, she learned the method to measure cytokines using ELISA and also CBA beads flowcytometrically. Dr. Chen made scientific presentation at the 15th annual meeting of Japanese Society of Immunotoxicology held at Towerhall Funabori, Tokyo, on September, 11 and 12, 2008. Her presentation was entitled "Comparison of cytokine secretion between a human T cell line and a subline continuously exposed to chrysotile asbestos". During the one year period as research fellow, Dr. Chen found low-dose and continuously exposed human CD4+25+ T cell line showed enhancement of regulatory function. This finding is very important to consider the immunoomdulation of asbestos in the view point of reduction of tumor immunity. I, Prof. Otsuki, promise we will write scientific manuscript with the data which Dr. Chen has found with some expansion of the analyses. I hope we will make Dr. Chen as the first author of the manuscript. I confirm Dr. Chen did her best and worked hard, and got very wonderful results to be the base of laboratory's main project. In addition, it should be added to this report that all the member of Otsuki's laboratory respect her works and love her characteristics.